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	Provedor de dados BJM (2) BABT (1) Autor Sato,Hélia H. (2) Chauhan,Anjali (1) Ferracini-Santos,Luciana (1) Fleuri,Luciana F. (1) Guleria,Shiwani (1) Kawaguti,Haroldo Y. (1) Mehta,Preeti (1) Shirkot,C.K. (1) Walia,Abhishek (1) Mais... Palavra-chave Cellulosimicrobium cellulans (3) Actinomycete (1) Alkaline protease (1) Cellulase free xylanase (1) Chitinase (1) Cloning (1) E.coli (1) Fungal lysis (1) Gene (1) Medium optimization (1) Protoplasts (1) Response surface (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2015 (1) 2009 (2) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Molecular Cloning and Sequencing of AlkalophilicCellulosimicrobium cellulans CKMX1 Xylanase Gene Isolated from Mushroom Compost and Characterization of the Gene Product BABT Walia,Abhishek; Mehta,Preeti; Guleria,Shiwani; Chauhan,Anjali; Shirkot,C.K.. ABSTRACT A xylanolytic bacterium was isolated from mushroom compost by using enrichment technique. Results from the metabolic fingerprinting, whole-cell fatty acids methyl ester analysis and 16S rDNA sequencing suggested the bacterium to be Cellulosimicrobium cellulans CKMX1. Due to the xylanolytic activity of this bacterium, isolation and characterization of the xylanase gene were attempted. A distinct fragment of about 1671 bp was successfully amplified using PCR and cloned into Escherichia coli DH5α. A BLAST search confirmed that the DNA sequence from the amplified fragment was endo-1, 4-beta-xylanase, which was a member of glycoside hydrolase family 11. It showed 98% homology withCellulosimicrobium sp. xylanase gene (Accession no. FJ859907.1) reported... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase free xylanase; Cellulosimicrobium cellulans; E.coli; Cloning; Gene. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600913 Production of alkaline protease from Cellulosimicrobium cellulans BJM Ferracini-Santos,Luciana; Sato,Hélia H.. Cellulosimicrobium cellulans is one of the microorganisms that produces a wide variety of yeast cell wall-degrading enzymes, β-1,3-glucanase, protease and chitinase. Dried cells of Saccharomyces cerevisiae were used as carbon and nitrogen source for cell growth and protease production. The medium components KH2PO4, KOH and dried yeast cells showed a significant effect (p<0.05) on the factorial fractional design. A second design was prepared using two factors: pH and percentage of dried yeast cells. The results showed that the culture medium for the maximum production of protease was 0.2 g/l of MgSO4.7H2O, 2.0 g/l of (NH4)2SO4 and 8% of dried yeast cells in 0.15M phosphate buffer at pH 8.0. The maximum alkaline protease production was 7.0 ± 0.27 U/ml... Tipo: Info:eu-repo/semantics/article Palavras-chave: Response surface; Medium optimization; Alkaline protease; Cellulosimicrobium cellulans; Actinomycete. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100008 Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191 BJM Fleuri,Luciana F.; Kawaguti,Haroldo Y.; Sato,Hélia H.. This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25ºC and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25ºC and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts. Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Cellulosimicrobium cellulans; Fungal lysis; Protoplasts. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300026 Registros recuperados: 3 Primeira ... 1 ... Última

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